The following is a list of episodes from the 31st and final season of PBS Mister Rogers' Neighborhood which aired in 2001. This season was the shortest of the entire run, with only five episodes before the death of Fred Rogers in 2003.
- Episode 1 (season premiere) Trees and Seeds/Prince Upset About His Drawing (August 27, 2001)
- Episode 2 Art with Sand/Arts Festival In Make-Believe (August 28, 2001)
- Episode 3 Native American Potter/Lady Elaine to Judge Arts Festival (August 29, 2001)
- Episode 4 Glass Harmonica/Quilts/Everyone's Art Is Unique (August 30, 2001)
- Episode 5 (series finale) How We Feel About Ourselves Affects How We Treat Others (August 31, 2001)
- This was the last season to have the PBS Kids 1999 logo with Dot and Dash.